In Vitro and Ex Vivo Mechanistic Understanding and Clinical Evidence of a Novel Anti-Wrinkle Technology in Single-Arm, Monocentric, Open-Label Observational Studies

Skin aging is a biological process leading to visible skin alterations. The mechanism of action, clinical efficacy and tolerance novel anti-wrinkle technology were evaluated in two care products formulated for different types. Two single-arm monocentric, open-label observational studies, which 56 days long, cream-gel (n = 30) cream 33) on the face neck. Morphometric analyses five types wrinkles performed at 0, 7, 28 days. Structural changes extracellular matrix (ECM) including collagen, elastin hyaluronic acid (HA) visualized quantified by histochemical imaging after daily treatment explants 6 Protein gene expression related barrier hydration analyzed using ELISA qRT-PCR, respectively, reconstituted human model treated 48 h. A decrease wrinkle dimensions was found majority parameters treatment. Collagen, elastin, HA, procollagen type I, hyaluronan synthases, HAS2 HAS3 all stimulated. Based significant consistent our investigations, we conclude that underlying action could be remodeling dermal ECM, both test formulations efficacious well tolerated.